The barrier membranes for GTR procedure could be affected by bacterial
contamination after exposure to oral environment.
This study was done to evaluate whether the tetracycline impregnated barrier
membranes could inhibit bacterial attachment and penetration into membranes. The
resorbable membrane(polylactic and polyglycolide copolymer, Resolute ,
W.L Gore and Associates, Inc., USA) and the non-resorbable membranes(e-PTFE,
Gore-TexTM, W.L. Gore & Associates, Inc. USA) were cut into 4mm discs and trated
with 5% tridodecylmethylammonium chloride solution in ethanol and dried in air. The
membranes were immersed in tetracycline ( TC ) solution(l00mg/ml, pH 8.0) and dried.
To the maxillary canine-premolar region in six periodontally healthy volunteers,
removable acrylic devices were inserted, on which 8 cylindrical chambers were glued
with TC impregnated and non-impregnated discs, the membrane discs vere examined for
bacterial attachment and penetration, and structural changes under SEM and .M.
From the 1st day to the 7th day, membranes showed bacterial plaque formation
composed of occi and rods. Thereafter, filamentous bacteria appeared and the plaque
thickness increased. The TC impregnated e-PTFE membranes showed less bacterial
attachment and delayed in bacterial laque maturation than non-treated membranes.
As for bacterial penetration, the TC impregnated e-PTFE membranes showed
superficial invasion and infrequent presence of bacteria in unexposed inner surface at the
4th week, while e non-treated e-PTFE membranes showed deep bacterial invasion at
the 2nd week and equent presence of internal bacteria at the 4th week. The resorbable
membranes started to be sorbed at the 2nd week and were perforated at the 4th week,
regardless of TC treatment.
In conclusion, bacterial plaque formation and penetration was efficiently delayed in
TC impregnated e-PTFE membranes, whereas resorbable membranes were similar in
bacterial vasion due to membrane degradation and perforation, regardless of TC
treatment.
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